Biochemistry Protocols

Biochemistry, Molecular Biology, and Cell Biology Protocols  >> Free Biochemistry Protocols

Protocol for the Ammonium Sulfate Precipitation of Proteins

DEAE Anion Exchange Protein Purification Protocol

Protocol for Dialysis of Protein Solutions

Immunocytochemistry and Immunofluorescence Staining Protocol

In-gel Digestion Protocol for Mass Spectrometry

How to Perform and Analyze a Sequence Alignment

How to Run Native Protein Gels

Solubilizing Membrane Proteins

Coomassie Blue Staining of SDS-PAGE

Solubilizing Membrane Proteins Protocol

Sedimentation Velocity Protocol

Gel Filtration Chromatography Protocol

Far Western Blot Protocol

ELISA Protocol (Enzyme-Linked Immunosorbent Assay)

Immunoprecipitation Protocol

SDS Gel Electrophoresis and Western Blot Protocol

Using HEPES Buffer in Biochemistry and Molecular Biology Experiments

Using Phosphate-Buffered Saline (PBS) in Biochemical and Cell Biology Research

Using Tris Buffer in Biochemistry and Molecular Biology Experiments

Biotinylation Protocol for Proteins or Peptides

Detecting Biotinylated Proteins with Streptavidin

Western Dot Blot Protocol

Reducing Proteins or Peptides with Dithiothreitol (DTT)

Alkylating Proteins or Peptides with N-ethylmaleimide (NEM)

Negative Staining Protocol for Transmission Electron Microscopy

 

 

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