Biochemistry, Molecular Biology, and Cell Biology Protocols
Free Biochemistry, Molecular Biology, and Cell Biology Protocols
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Splitting and Passaging Cells Protocol - How to Passage Cells
Transfecting Adherent Cells in Suspension
Running DNA Agarose Gels - How to Run a DNA Agarose Gel
Protocol for the Ammonium Sulfate Precipitation of Proteins
DEAE Anion Exchange Protein Purification Protocol
Protocol for Dialysis of Protein Solutions
Restriction Enzyme Digestion of Plasmid DNA
Growing Bacterial Cultures
Immunocytochemistry and Immunofluorescence Staining Protocol
Immunoprecipitation Pull-Down Assay Protocol Using Protein A or Protein G Beads
In-gel Digestion Protocol for Mass Spectrometry
Performing DNA Ligations for Molecular Cloning - Ligation Protocol
Cell Fractionation Protocol
Molecular Cloning Tips
Designing Primers - A Checklist
Polymerase Chain Reaction (PCR) Protocol
Solubilizing Membrane Proteins with Triton X-100 Protocol
Protocol on How to Solubilize Membrane Proteins with the CHAPS Detergent
Sedimentation Velocity Protocol
Gel Filtration Chromatography Protocol
How to Perform a Gel Extraction
Site-Directed Mutagenesis Protocol Using PCR
Tips on Using the Cell Culture Room
Cell Culture - Freezing, Thawing, and Adapting Cells
Making Agar Plates and Doing Transformations
Co-Immunoprecipitation (co-IP) Protocol on How to Find Protein-Protein Interactions
Producing Reporter or Chimeric Fusion Proteins - How to Make a Chimeric or Reporter Fusion Protein
How to Perform and Analyze a Sequence Alignment
Far Western Blot Protocol
How to Produce a Stable Cell Line
Coomassie Blue Staining of SDS-PAGE
ELISA Protocol to Detect Protein Interactions- Enzyme-Linked Immunosorbent Assay
Silver Staining of SDS Polyacrylamide Gels
How to Run Native Protein Gels
SDS Gel Electrophoresis and Western Blot Protocol
Subcloning Protocol
Generation of an Affinity Column with Sepharose
Biotinylation Protocol for Proteins or Peptides
Using Bovine Serum Albumin as a Blocking Agent
Western Dot Blot Protocol
Reducing Proteins or Peptides with Dithiothreitol (DTT)
Producing a Flag-Tagged Protein
How to Infect Mammalian Cell Cultures with Viruses
Negative Staining Protocol for Transmission Electron Microscopy
Alkylating Proteins or Peptides with N-ethylmaleimide (NEM)
Detecting Biotinylated Proteins with Streptavidin
Using HEPES Buffer in Biochemistry and Molecular Biology Experiments
Separating the Supernatant and Solid Phases of a Sample by Microcentrifugation
Running a Urea Gel for Northern Blot Analysis
Using Tris Buffer in Biochemistry and Molecular Biology Experiments
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