Biochemistry, Molecular Biology, and Cell Biology Protocols

Free Biochemistry, Molecular Biology, and Cell Biology Protocols

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Splitting and Passaging Cells Protocol - How to Passage Cells

Transfecting Adherent Cells in Suspension

Running DNA Agarose Gels - How to Run a DNA Agarose Gel

Protocol for the Ammonium Sulfate Precipitation of Proteins

DEAE Anion Exchange Protein Purification Protocol

Protocol for Dialysis of Protein Solutions

Restriction Enzyme Digestion of Plasmid DNA

Growing Bacterial Cultures

Immunocytochemistry and Immunofluorescence Staining Protocol

Immunoprecipitation Pull-Down Assay Protocol Using Protein A or Protein G Beads

In-gel Digestion Protocol for Mass Spectrometry

Performing DNA Ligations for Molecular Cloning - Ligation Protocol

Cell Fractionation Protocol

Molecular Cloning Tips

Designing Primers - A Checklist

Polymerase Chain Reaction (PCR) Protocol

Solubilizing Membrane Proteins with Triton X-100 Protocol

Protocol on How to Solubilize Membrane Proteins with the CHAPS Detergent

Sedimentation Velocity Protocol

Gel Filtration Chromatography Protocol

How to Perform a Gel Extraction

Site-Directed Mutagenesis Protocol Using PCR

Tips on Using the Cell Culture Room

Cell Culture - Freezing, Thawing, and Adapting Cells

Making Agar Plates and Doing Transformations

Co-Immunoprecipitation (co-IP) Protocol on How to Find Protein-Protein Interactions

Producing Reporter or Chimeric Fusion Proteins - How to Make a Chimeric or Reporter Fusion Protein

How to Perform and Analyze a Sequence Alignment

Far Western Blot Protocol

How to Produce a Stable Cell Line

Coomassie Blue Staining of SDS-PAGE

ELISA Protocol to Detect Protein Interactions- Enzyme-Linked Immunosorbent Assay

Silver Staining of SDS Polyacrylamide Gels

How to Run Native Protein Gels

SDS Gel Electrophoresis and Western Blot Protocol

Subcloning Protocol

Generation of an Affinity Column with Sepharose

Biotinylation Protocol for Proteins or Peptides

Using Bovine Serum Albumin as a Blocking Agent

Western Dot Blot Protocol

Reducing Proteins or Peptides with Dithiothreitol (DTT)

Producing a Flag-Tagged Protein

How to Infect Mammalian Cell Cultures with Viruses

Negative Staining Protocol for Transmission Electron Microscopy

Alkylating Proteins or Peptides with N-ethylmaleimide (NEM)

Detecting Biotinylated Proteins with Streptavidin

Using HEPES Buffer in Biochemistry and Molecular Biology Experiments

Separating the Supernatant and Solid Phases of a Sample by Microcentrifugation

Running a Urea Gel for Northern Blot Analysis

Using Tris Buffer in Biochemistry and Molecular Biology Experiments

 

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