Tips on Using the Tissue Culture Room

Biochemistry, Molecular Biology, and Cell Biology Protocols  >> Tips on Using the Tissue Culture Room

Using the tissue culture room

keep the door closed to prevent contamination from the outside going into the room
turn on the fan in the hood ~15 minutes before use
wipe counter with ethanol before use
flame bottles, metal boxes for long pipets and pasteur pipets before and after use
when finished with aspirator, aspirate some 5% bleach to wash out the hose
wipe counter with ethanol when finished
if the 70% ethanol or 5% bleach is full, refill them; if the flask for emptying liquid is full, change it by adding bleach to the flask, rinsing with water, and running lots of water down the sink

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