Tips on Using the Tissue Culture Room

Biochemistry, Molecular Biology, and Cell Biology Protocols >> Tips on Using the Tissue Culture Room

Using the tissue culture room

· keep the door closed to prevent contamination from the outside going into the room
· turn on the fan in the hood ~15 minutes before use
· wipe counter with ethanol before use
· flame bottles, metal boxes for long pipets and pasteur pipets before and after use
· when finished with aspirator, aspirate some 5% bleach to wash out the hose
· wipe counter with ethanol when finished
· if the 70% ethanol or 5% bleach is full, refill them; if the flask for emptying liquid is full, change it by adding bleach to the flask, rinsing with water, and running lots of water down the sink