Cell Culture - Freezing, Thawing, and Adapting CellsBiochemistry, Molecular Biology, and Cell Biology Protocols >> Cell Culture - Freezing, Thawing, and Adapting Cells Freezing cells
Whenever have a new cell line completed (e.g. after stable selection, or receiving a new cell line, or after adaptation into different media) freeze away a few vials of stocks
Thawing cells
1. Thaw cells quickly in the 37-degree water bath to prevent them from dying (turn the tube in both directions in the water bath)
Adapting cells to new conditions(e.g. suspension culture, new media)
1. Split cells at twice the concentration of its lower splitting dilution (perhaps even a 1:2 split)
To convert from g to rpm use this formula:
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